The present investigation is to estimate biologically active flavonoidal compounds, quercetin and rutin in ethanolic leaves extract of Melia azedarach Linn by using high-performance thin-layer chromatography (HPTLC). Pre coated silica gel 60 F254 used as stationary phase and Toluene: Ethyl Acetate: methanol in ratio of 5: 3: 2 are used as mobile phase. Densitometric estimation and quantification of these compounds was carried out at 254nm. The standard Rf values of rutin and quercetin are 0.17 and 0.65 respectively. The total peak areas of the standards, quercetin and rutin were compared and the corresponding peak areas of extracts were estimated to be 1284.64 and 1037.27 respectively. This HPTLC method was found to be simple and convenient for rapid screening of active compounds and quantification of the investigated flavonoids in Melia azedarach Linn.