- Asian Journal of Biomedical and Pharmaceutical Sciences (2015) Volume 5, Issue 47

Simultaneous Estimation of Metformin Hydrochloride, Glimepiride and Pioglitazone using First Generation Chemometric Methods

In this study, new feasible UV-Visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of Metformin hydrochloride (MET), Glimepiride (GLI) and Pioglitazone (PIO) in combined pharmaceutical tablets. Methanol is used as a solvent for analysis and whole UV region has been scanned from 200-400 nm. The resolution has been obtained by using multivariate methods as Classical least square (CLS), Inverse least squares (ILS), Principal Component Regression (PCR), Partial Least Squares Regression (PLSR) applied to the UV spectra of the mixture. The results obtained from all the methods were found within the limit.

Author(s): Pragya Nand Badyal, Chetan Sharma, Ravi Shankar, Ravindra K. Rawal

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