Antioxidants had been proven to play an important role in the regulation of a vast array of physiological and pathological processes. They principally contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species and other free radicals. Plants play an important role in the maintenance of human health primarily via nutrition and also contribute greatly to the management of various ailments. The therapeutic potential of Indigofera tinctoria was evaluated by paracetamol induced hepatotoxicity in rats. Male albino Wistar rats were orally treated with Indigofera tinctoria (75, 150 and 300 mg/kg body weight) or silymarin (25 mg/kg) daily with the administration of paracetamol (3 gm/kg body weight- po) only one day. Paracetamol induced hepatotoxicity and significantly increased the activities of lipid peroxidation (TBARS), decreased the activities of superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) in kidney as compared with the control group. Treatment with Indigofera tinctoria or silymarin consecutively for twenty eight days could significantly decrease the activity of TBARS where as enhance the activities of SOD, CAT and GSH in the kidney when compared with paracetamol alone treated rats.