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- Asian Journal of Biomedical and Pharmaceutical Sciences (2012) Volume 2, Issue 11
Quantification of (-) Epicatechin by HPTLC Method in Developed Polyherbal Tablet Formulation
A simple sensitive HPTLC method developed for the Quantification of (-) epicatechin in the plant raw material tablet formulation. Traditional system of medicine recommends various hepatoprotective agents and preparations to treat hepatic disorders. Polyherbal crude drugs formulation (tablet) was developed by using well documented medicinal plants, Cassia fistula, Coccina indica and Vigna mungo for treatment of liver disorders by exploiting the knowledge of Traditional system of medicine. The stationary phase was precoated aluminium silica gel G F 254 Plates. The mobile phase for was chloroform: acetone: formic acid (75:16.5: 8.5). The plate was scanned and quantified at 364 nm for (-) epicatechin. The amount of (-) epicatechin was estimated by the comparing the peak area of standard and the same was present in the raw material tablet formulation. The content of (-) epicatechin was found to be 1.22% w/w in polyherbal crude tablet formulation. The calibration curve was linear in the range of 1 μg to 5 μg/spot and the correlation coefficient was found to be 0.9964. The limit of quantification was found to be 3 μg/spot and the limit of detection was 1 μg/spot. The method was validated in terms of precission and reproducible expressed as % RSD which were found to be less than 2%. The recovery values obtained were 98.28 to 100.4%, showing accuracy of the method. The average percentage recovery was found to be 99.12%. This estimation technique is very much useful for the estimation of (-) epicatechin present in the various medicinal plants and formulations.
Author(s): Praveen Patidar, Narendra Madoria, Sameer Singh, and Kamlesh Dashora