Urinary tract infections (UTI) cause a many morbidity and mortality. The aim of this study was to study the risk factors and uropathogens of urinary tract infection in all patients. In the present study 2400 patients with clinical symptoms and suspected to UTI were examined. Clean-catch midstream urine was collected. Urine samples were inoculated, isolated and identified using standard bacteriological methods. Over all 650 (27.1%) uropathogens isolated belonging to 12 species with four Gram-positive, seven Gram-negative bacteria and candida spp. during the span of 12 months. The overall prevalence of UTIs in women was 69.8 % and men was 30.2%. Age-wise distribution of uropatogens was predominant in the age groups of 20-30 years both women (105, 16.2%) and men (40, 6.2%). In almost of patients (60.9%) was associated with common urinary tract infection. Urinary cystis was the second (9.7%) leading risk factor causing urinary tract infection followed by urithritis (7.9%), catheterization (7.7%), pyelonephritis (4.3%) and suspected cancer (3.1%) almost equally contributed. In the gram Negative bacteria Escherichia coli were the predominant pathogen in both the groups (38.09%). Klebsiella Sps (11.2%) was the second common organism in hospital acquired infection followed by Pseudomonas sps. (6.8%), Proteus sps. (3.7%), Enterobacter sps. (0.6%), Citrobacter sps. (0.3%) and Acinetobacter sps. In the gram positive bacteria the main organism identified was Coagulase Negative Staphylococcus (4.1%), Enterococcus (3.7%), Staphylococcus saprophyticus, (2.3%), Staphylococcus aureus (1.1%), and candida species. In the present study, overall incidence of UTIs were observed in females. High rate of UTI was observed in female of 21-40 years age. It is concluded that Gram-negative bacilli were responsible for UTI infections in our patients. The common isolated bacteria from urinary tract infections were E.coli. In the Indian setting, routine urine cultures may be advisable, since treatment failure is likely to occur with commonly used antimicrobials. This study provides valuable laboratory data to monitor the status of uropathogens and to improve treatment recommendations in a specific geographical region.