Preliminary phytochemical screening is the key step for finding the chemical constituents which leads to the isolation of lead compounds of medicinal importance. Myxopyrum smilacifolium is a large woody climbing shrub belonging to the family Oleaceae. Its root, stem, leaves are of much medicinally active and is employed in many traditional systems of medicines. The main focus of this study is to identify and understand the bioactive chemical constituents of the root extracts by subjecting the root powder to soxhlet extraction in different solvent systems. The yield of each extract was calculated and it was found to be more in methanolic extract. The phytochemical screening showed the presence of alkaloids, phenolics, glycosides, tannins, flavonoids etc which contribute several pharmaceutical applications to humans.