In developing countries like India, low income people such as farmers, isolated villagers and native communities are using folk medicines for the treatment of common infections. Herbs and spices are known to produce certain bioactive compounds which react with other organisms in the environment. Phytochemicals are known to be biologically active and they are formed to contain various biomolecules effective against many diseases and disorders. In the present work, dried leaf powered of Murraya koenigii was subjected to phytochemical screening in aqueous extract and antimicrobial activity using four different organic solvents like aqueous, acetone, isopropanol and methanol against three pathogenic bacteria such as Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus by disc diffusion method. The phytochemical compounds were qualitatively analysed and the antimicrobial activity of Escherichia coli and Staphylococcus aureus showed maximum zone of inhibition in acetone extract, whereas Klebsiella pneumonia showed maximum zone of inhibition in aqueous extract.