The experiments of Pharmacognostical standardization in fresh, powdered plant material and anatomical sections of the root of Zyziphus oenoplia (L.) Mill was carried out to determine its morphoanatomic and microscopical characters and also some of its quantitative physiochemical standards. Roots were cylindrical, blackish brown surface, longitudinal striations with irregular transverse ridges with splintery- fibrous fracture and yellowish brown in colour. Internally roots show the presence of cork, epidermis, periderm, vessels, cortex, phloem, xylem, endodermis and starch grains. The powder microscopy revealed the presences of Fibres and other cell structures. Physicochemical investigation includes ash values, extractive values and moisture content, swelling index, foaming index was evaluated. These present investigation will be useful towards establishing pharmacognostic standards on which helps batter in understanding their identification, purity, quality and medicinal importance in depth which can provide the reliable information for further pharmaceutical applications and research.