Asian Journal of Biomedical and Pharmaceutical Sciences

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Occurrence of Extended Spectrum Beta - Lactamase Producing Enterobacteriaceae Causing Wound Infections

At present scenario, the extended spectrum beta lactamase (ESBL) producing bacterial pathogen causes various life threatening infections lead to sepsis related mortality. A wide variety of microorganisms, like Staphylococcus aureus, Pseudomonas aeruginosa, and Enterobacteriaceae-like Klebsiella pneumoniae and Escherichia coli, are involved. Extended-spectrum betalactamases (ESBLs) constitute a growing class of plasmid-mediated ßlactamases which confer resistance to broad spectrum beta-lactam antibiotics. Resistance is generally increasing, with reports of multidrugresistant isolates. This study was conducted to determine the ESBL producing bacterial isolates in our setting and describe their antimicrobial susceptibility. In the present investigation, 110 samples were screened on different bacteriological media. Wound samples were collected from different hospital of city. Antimicrobial susceptibility tests were done using disc diffusion technique as per the standard of Kirby-Bauer method. Bacterial pathogen identity was confirmed based on standard methods which included, Gram stain reaction, colonial morphology on media, lactose fermentation, catalase, oxidase, coagulase and indole tests. Out of 110 wound swab samples analyzed, 90 (87.8%) were culture positive. From 90 bacterial isolates, the bacteria were identified as Pseudomonas aeruginosa was the most frequently isolated organism 46.66%, followed by E. coli (24.44%), Klebsiella spp. (22.22%), Proteus spp. (8.88%) The antibiotic sensitivity test were shown that Pseudomonas spp. was mostly resistant to ampicillin (90.47%) and tetracycline (78.57%). E.coli showed the highest resistance to Ampicillin, Amikacin, cefotaxime and tetracycline were 90.90%, 86.36%, 81.81% and 72.72% respectively. Klebsiella spp. showed highest resistance to Ampicillin and gentamicin( 83.33%), Amikacin, cefotaxime, cotrimoxazole showed 77.77% resistance. Proteus spp. had resistance rate of 100% to Ampicillin and Gentamicin. Amikacin and ceftriaxone showed 87.5% resistance to antibiotics. ESBL production among these isolates was checked by combination disc method and about 72% isolates were found to be ESBL producers. So our result indicates the fact that the physicians should be aware of this increasing resistance among our local clinical isolates and should change their therapy regime accordingly.

Author(s): P. M. Tumane, Durgesh D. Wasnik