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Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2017) Volume 7, Issue 62
Influence of the Zhichan capsule on the Expression of TNF-?, IL-1?, IL-6 and T, B Lymphocytes Function in PD Mice
Objective: To investigate the immunomodulatory effect of the Zhichan capsules on the Parkinson's disease (PD) induced mice. Methods: Intraperitoneal injection of MPTP induced in a PD model, reverse transcription polymerase chain reaction (RT-PCR)is performed to detect the gene expression of Interleukin 1 beta (IL-1β), IL-6 and Tumor Necrosis gene Factor (TNF-α); the lymphocyte transformation test and haemolytic plaque assay was done to analyse the effect of Zhichan capsules on T, B lymphocytes proliferation. Results: The expression of IL-1β and TNF-α mRNA were increased, and the IL-6 was reduced in PD model control group compared with normal control group. The expression of IL-1β and TNF-α mRNA were reduced, and the IL-6 was increased in the treatment group which were treated with the Zhichan capsules compared with the PD model control group, and the expression of them had significant differences (p<0.05); compared with the control group, the transformation rate of T cells and the number of plaques formed in treatment group were significantly increased, they both have statistical significance (p<0.05). Conclusion: Zhichan capsules have a down-regulation on the expression IL-1 β and TNF-α mRNA and an up-regulation on IL-6 and the T, B lymphocytes in PD mouse. It was indicated that Zhichan capsules have preventive and therapeutic effect on PD.
Author(s): Yongmao Liu, Pengguo Zhang, Yanling Wang, Shuang Wu, Lin Tian, Qingwei Zhou