Hypertension is a major health problem in developed as well as in developing countries. It is one of the most important public health challenges worldwide because of its high frequency and concomitant risks of cardiovascular and kidney disease. The purpose of this study was to compare the blood lipid levels in hypertensive subjects with normotensive subjects by making the association of hypertension with lipid profile in hypertensive cases. This prospective study was carried out in general medicine and pathology departments of Govt. Hospital in Rishikesh. In the present study, the volunteers were selected from General Medicine department of Government hospital Rishikesh from May 2009 to May 2010. Hypertension was defined as per the recommendations of JNC7th Report. After 12 hours fast the blood samples were collected from all individuals without anticoagulant and centrifuged at 3000 rpm for 5 minutes. The serum was collected in fresh vial and standard methods were followed for biochemical studies. A total of 120 volunteers were recruited for this study. Out of them 70 were hypertensive subjects (38 males and 32 females) and 50 were normotensive subjects (27 males and 23 females). The results concluded that total cholesterol, LDL- C, VLDL-C and triglycerides were higher and statistically significant in hypertensive subjects than normotensive subjects (p<0.05). The mean HDL-C in hypertensive subjects was lower than normotensive subjects and statistically significant (p< 0.05).