India is endowed with a rich wealth of medicinal plants. Approximately 80% of world population still relies on traditional medicines for the treatment of common illnesses. Rural people were more susceptible for diseases but they couldn’t afford synthetic drugs due to their higher import cost. Although large numbers of synthetic antimicrobial agents have been discovered, they have side effects and pathogenic microbes are constantly developing resistance to these agents. Cassia glauca leaves and stem are traditionally claimed for various biological activities, and the plant is being utilized without any scientific validation. Hence, in the present study leaves and stems of C. glauca have been evaluated for their antibacterial activity against the disease causing organism S. aureus, E. coli, E. faecalis and Klebsiella and antifungal activity against A. fumigatus and C. albicans using petroleum ether, ethanol by serial dilution method. All the extracts of C. glauca leaf and stem were found to be more potent anti-bacterial against S. aureus, and E. faecalis (MIC< 0.195 mg/ml) when compared Klebsiella and E. coli. (MIC= 0.391 – 100 mg/ml). They also exhibited potent anti-fungal activity against the C. albicans (MIC< 0.195 mg/ml) and A. fumigates (MIC 0.195 –1.562 mg/ml). Thus the result of present work supports the traditional use of Cassia glauca and can be further explored for their potential therapeutic use.