Lepidium draba, a weed of the Brassicaceae family contains high level of glucoraphanin that can be produced sulforaphane (SFN) through myrosinase hydrolysis activity. This study was conducted to optimize conditions for SFN production in this plant; several factors including age of seedlings and calluses as well as application of different kind of elicitors such as methyl jasmonate and salicylic acid (pH=7), and also Cu2+ and Zn2+ (pH=5 and 7) were examined. The results showed that the highest amount of SFN was achieved in 7-day-old seedlings and 14-day-old calluses, but more significantly in the seedlings. In general, elicitation of the 7-day-old seedlings was associated with a clear improvement in SFN content, but more drastically with metals-treated under acidic condition. In the metals-treated seedlings, it seems that the promotion of SFN content is due to the more adsorption of the ions under the acidic condition and subsequently induced the glucoraphanin biosynthesis pathway.