The present investigation was aimed to isolate and assess the bacterial samples from the rhizosphere soil. Out of twenty strains, four strains were assessed for their probiotic activity towards the growth of fish in the particular habitat. The experiments were conducted by admixing the strain with water and fortified with the commercial feed. Results pertaining to the present investigation clearly revealed that only one strain (MA4 -Antagonistic effect) was showed better activity. It was identified as Bacillus sp. Identified strain was examined for its effect on fish growth in the form of probiotics. Furthermore, the bacterial strain mixed in water enhanced the water qualities and also significantly increased the length and weight of freshwater fish, Labeo rohita.