The most of drug-drug interaction between oral hypoglycemic agents and antifungal agents was reported in healthy rats, rabbits and diabetic rats. The aim of this study was evaluated the effect of itraconazole on therapeutic efficacy of rosiglitazone in healthy albino rats. The blood was withdrawn from the retro-orbital plexuses of rats at different time intervals at 0hr, 1, 2, 4, 8, 12, 18 and 24 hour and blood glucose was estimated by GOD-POD enzymatic method and values are expressed in mg/dl. The results indicate itraconazole enhances the onset, peak effect and duration action of rosiglitazone in healthy rats during concomitant administration of these drugs. In conclusion, the dose and frequency of administration of itraconazole and rosiglitazone should be readjusted while giving simultaneously.