This study was designed to investigate the effect of Clonidine on lipid profile parameters like total cholesterol, High density lipids, triglycerides, low density lipids in alloxan-induced diabetic rabbits. Rabbits were divided into six experimental groups: Nondiabetic control group treated with normal saline, Nondiabetic test group treated with Clonidine, diabetic control group treated with normal saline, diabetic test group treated with Clonidine, diabetics treated with Metformin, diabetics treated with Metformin & Clonidine. Treatment with drugs was started on the 3rd day of alloxan treatment (i.e. day 1) and was continued for 30 days. Statistical evaluation was done using student’s ‘t’ test & one way ANOVA followed by Tuckey`s post hoc test. ‘p’ value less than 0.05 was considered statistically significant. But clonidine does not affect any lipid levels in both euglycemic & diabetic rabbits as the p value was found to be statistically insignificant i.e. more than 0.05.