Background: Smoking is one of the environmental factors which can alter normal lipid profile. It is one of the major risk factors in the genesis of coronary atherosclerosis and development of coronary heart disease. The present study aimed to compare the lipid profile between smoker and non smoker and to evaluate the durational significance on lipid profile in the smokers and to clarify the influence of daily cigarette smoking on the components of lipid profile in Sudanese youth males. Materials and Methods: Out of 200 apparently healthy male subjects, 100 were smokers [26.2±5.2Years] and 100 were non smokers [24.0±4.6Years]. The subjects were asked to fast overnight and early morning blood samples collected and analyzed for lipid profile [Cholesterol, Triglyceride, High density lipoprotein and Low density lipoprotein] by using Cobas integra 400 plus Roche analyser. Results: A significantly increased of serum Cholesterol, Triglyceride and Low density lipoprotein with significant decreased in serum High density lipoprotein level in smokers as compared to non smokers and same results were found in smoker group with > 15 cigarettes smoked per day while with increase duration of smoking the TC & LDL-C were increased, TG showed no difference while the HDL-C was decreased showing greater risk of these persons to atherosclerosis and coronary heart disease. Conclusions: This study concluded that smoking causes alteration in lipid profile. Increased duration of smoking and number of smoked cigarettes / day causes more dyslipidaemia .This smoking might be related in the alteration in serum lipid levels increases risk for coronary artery disease.