Hydrolytic enzymes activities in digestive tract of grasscutter were measured on 40 adult grasscutters. The selected animals were apparently healthy and adulte. Each portion of digestive tract and the contents was recovered in a jar which contained physiological water. Amylolytic, cellulolytic and invertasic activities were measured at pH 2.6, pH 3, pH 4, pH 5, pH 5.6, pH 7, pH 8 and pH 9 in each portion of digestive tract. Only the proteolytic activity was measured at 1.7, 7, 9 and 10. Enzymatic activities mixture showed that amylolytic, cellulolytic, invertasic and proteolytic activities changed according to pH and portions of digestive tract. The amylolytic activity is dispersed in all digestive contents, glands and digestive muscle excepted the oesophagus. Cellulolytic activity is detected in the digestive contents, precisely in the caecum. An important proteolytic activity was detected in the pancreas and no xylanasic activity was detected in tissues and digestive contents.