Asian Journal of Biomedical and Pharmaceutical Sciences

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Development of New Analytical Method and Its Validation for the Determination of Tramadol Hydrochloride in Bulk and Marketed Formulations

Tramadol hydrochloride in presence of acidic medium reacts with excess amount of Chloramine-T and remaining Chloramine-T react with Crystal violet to produce blue colour. The final stock solution was made to produce 100μg/ml with methanol. The λmax was found to be 591 nm for assay. The linearity was found in concentration range of 5-30μg/ml. The correlation coefficient was found 0.999. The regression equation was found as Y= 0.025X + 0.001.The method was validated according to ICH Guidelines.

Author(s): Sagarbechara, Padmavathi P. Prabhu, Subrahmanyam E.V.S, Jignesh J. kaneria, Sumit saparia, Shabaraya A.R