The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefixime trihydrate in bulk and pharmaceutical formulation. The solvent used throughout the experiment was the mixer of methanol and water. Absorption maximum (λmax) of the drug was found to be 287 nm. The quantitative determination of the drug was carried out at 287 nm and Beer’s law was obeyed in the range of 2-20μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.025x + 0.078 with correlation coefficient of 0.999. The recovery values for cefixime trihydrate ranged from 99.57% - 100.86%. The relative standard deviation of six replicates of assay was less than 2%. The percent relative standard deviation (RSD%) of interday precision range was 0.059 – 0.546 % and intraday precision range was 0.102 – 0.299%. The limit of detection and limit of quantification was 0.053 μg/mL and 0.159 μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.258 - 0.365%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.