Urinary tract infections are among the most common infectious diseases in humans. The incidence of candidiasis is on the rise in hospitalized patients especially due to non-albicans Candida. The present study was aimed to isolate, identify, and perform antifungal susceptibility testing of the yeast isolates from the urine samples. The urine samples from a total of 2400 patients in the Pattukkottai over a period of 18 months were obtained. Candida species were isolated from twenty two patients. The prevalence candiduria in critically ill patients in our diagnostic centre was 3.4 percent. Females were affected predominantly (81.8%). The species were identified according to morphology and color of colonies on Chromagar Candida. C. albicans (45.5%) was the highest occurring pathogens isolated, followed by C. glabrata (22.7%), C. tropicalis (18.2%), and C. krusei (13.6%). The antifungal sensitivity tests carried out using commercially available antifungal disc. Amphotericin B and itraconazole 21 (95.5%) was found to be the most effective antifungal agent, followed by ketoconazole, and fluconazole.