Biological synthesis of nanoparticles has attracted attention due to its inherent advantages over the physical and chemical methods of nanoparticle synthesis. The greener way of the synthesis attributes to avoid use of the hazardous chemicals and their subsequent effects. In this investigation, we have used plant seed extract of Pisum sativum to develop safe, reliable and eco-friendly process for the synthesis of silver nanoparticles. This approach showed that seed extract of Pisum sativum reduced silver nitrate into the formation of silver nanoparticles. The generated silver nanoparticles were characterized by using UV–vis spectroscopy, Transmission electron microscopy (TEM) and Fourier transform spectroscopy (FTIR). The formation of silver nanoparticles was confirmed by UV-vis spectrophotometer. The UV-vis spectra showed peak at 423.02 nm. TEM image showed that generated nanoparticles were spherical in shape with size range of 3-36 nm. The generated silver nanoparticles revealed antimicrobial activity by well diffusion method against Escherichia coli and yeast Candida albicans.