Rice (Oryza sativa L.) is the world’s single most important food crop. Rice is one of the few crop plants with rich genetic diversity, having a great demand of increased productivity. Genetic diversity is the key to overcome to biotic and abiotic stresses, so exploitation of germplasm diversity is the only solution. Thus, present study was planned to characterize the rice biodiversity of a set of thirty rice accessions collected from IRMBP. Protein was extracted from these rice seed using easy and user friendly protocol and SDS-PAGE. There were 7 major protein bands present across all accessions, medium mobility of them present in all accessions while, and remaining low mobility bands we found polymorphic. The similarity coefficient was analyzed using NTSYS-pc program, which ranged from 0.42 to 1.00. Biochemical based marker (Protein) was efficient for the assessment of genetic diversity. All the accessions were different and genetically distant from each other at the biochemical level. The results of the present study as the representative accessions of IRMBP led to a conclusion that the rich biodiversity still exists and should be explored for allele mining, and rice improvement programs.