The present study was undertaken to investigate Anti-Staphylococcus aureus and Anti-yeast activity of ethyl acetate extracts from six Streptomyces species (SSC-MB-01 to SSC-MB-06) isolated previously from rhizosphere soil of Sahyadri Science College campus, Shivamogga. Antibacterial activity was performed by agar well diffusion assay against four isolates of Staphylococcus aureus (resistant to methicillin) recovered previously from burn patients. Anti-yeast activity was checked against two yeasts viz., Candida albicans and Cryptococcus neoformans by agar well diffusion assay. Extracts of all Streptomyces species have shown inhibition of yeasts and clinical isolates of S. aureus. Among six isolates, SSC-MB-04 caused highest inhibition of S. aureus isolates. Among yeasts, higher susceptibility was recorded in case of C. neoformans. The Streptomyces species from the campus soil have shown to be promising against S. aureus and human pathogenic yeasts. Further studies on separation of bioactive principles from the extracts and determination of their bioactivities are under progress.