Background: Toxigenic strains of Escherichia coli are common enteric pathogens of human. The aim of this study was to detect virulence genes and antibiotic resistance pattern of the enterotoxigenic and enteroaggrigative E. coli isolated from diarhoeal stool samples. Methods: Totally, 234 diarrhoeal stool samples were collected. Microbiological examinations were done to detect the E. coli. PCR was used to identify Lt, Sta, Stb and East1 genes. Antibiotic resistance test was performed using the Disk diffusion method. Results: Out of 114 isolated E. coli, 15(13.5%) harbored Stb, 52(45.61%) East1, 30 (26.31%) Lt gene. The Sta gene was not detected in tested samples. The lowest resistance was for gentamicin (0%) while the highest resistance was for trimethoprim (79.8%). Resistance of E. coli isolates to chloramphenicol, cephotaxime, sulphametoxazole, ciprofloxacin, ampicillin and tetracycline were 3.5%, 7.01%, 71.05%, 10.5%, 52.63% and 3.5%, respectively. Conclusions: Toxigenic E. coli strains perticipated in diarrhoea in Shahrekord- Iran. The high presences of antibiotic resistance have been shown to trimethoprim, sulphametoxazole and ampicillin.