Endodontic treatment is easily accomplished by the complete removal of the infected nerve of the tooth i.e. diseased pulp and replacing it with the certain specific material. Thus, success or failure of the treatment can be evaluated by proper cleaning & shaping of root canal. Root canal treatment is used to save teeth which would otherwise need to be removed. It is needed when the blood or nerve supply of the tooth known as the pulp is infected through decay or injury. During this treatment one of the major problems is that the patient suffers with a lot of pain and inflammation at the site of injury. To overcome these problems self developed root canal irrigant in the form of gel were employed which posses a good analgesic and anti-inflammatory activity. In the present study efforts were made to formulate root canal irrigant having analgesic and anti-inflammatory activity. The self developed root canal irrigant was evaluated for Anti-inflammatory and Analgesic activities. The result of this study revealed that, the self developed root canal irrigant produced significant Anti-inflammatory and Analgesic activities. Indomethacin and Diclofenec sodium were used as standard drug for antiinflammatory and analgesic activities respectively.