pharma-summit-2019-scientifictracks

Note:

Page 14

July 18-19, 2019 | Valencia, Spain

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

Asian Journal of Biomedical and Pharmaceutical Sciences

ISSN: 2249-622X | Volume 9

Pharma Summit 2019

GLOBAL PHARMA SUMMIT

STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMI-

NATION OF ALPRAZOLAM, CLIDINIUM BROMIDE AND DICYCLOMINE HYDROCHLORIDE

BY RP-HPLC

Lal Prasanth M L

DMWIMS College of Pharmacy, India

simple, accurate, precise method was developed for the simultaneous estimation of the Dicyclomine hy-

drochloride, Clidinium bromide and Alprazolam in Tablet dosage form. Chromatogram was run through

Kromasil 250 x 4.6 mm, 5m. Mobile phase containing buffer and Acetonitrie in the ratio of 40:60 was pumped

through column at a flow rate of 1ml/min. Buffer used in this method was OPA buffer at pH 2.8. Tempera-

ture was maintained at 30°C. Optimized wavelength for Dicyclomine hydrochloride, Clidinium bromide and

Alprazolam was 225nm. Retention time of Dicyclomine hydrochloride, Clidinium bromide and Alprazolam

were found to be 2.473min, 3.842min and 5.218min. %RSD of system precision for Dicyclomine hydrochloride,

Clidinium bromide and Alprazolam were and found to be 0.3, 0.7 and 1.2 respectively. %RSD of method preci-

sion for Dicyclomine hydrochloride, Clidinium bromide and Alprazolam were and found to be 0.9, 0.5 and 0.5

respectively. Percent recovery was obtained as 99.86%, 100.33% and 100.45% for Dicyclomine hydrochloride,

Clidinium bromide and Alprazolam respectively. LOD, LOQ values are obtained from regression equations of

Dicyclomine hydrochloride, Clidinium bromide and Alprazolam were 0.41ppm, 0.03ppm, 0.005ppm, 1.24ppm

and 0.10ppm, 0.01ppm respectively. Regression equation of Dicyclomine hydrochloride was y= 8451x+13349,

Clidinium bromide was y= 13323x+6412 and of Alprazolam was y= 90717x + 3843.

Lal Prasanth M L, Asian J Biomed Pharmaceut Sci 2019, Volume 9

Lal Prasanth M L is from DM WIMS College of Pharmacy, India. His research findings mainly focus on stability indicating method

development and validation for the determination of Alprazolam, Clidinium bromide and Dicyclomine hydrochloride by RP-HPLC.

BIOGRAPHY