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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4
th
International Conference on
A novel immobilized method for isolation of phage display library-derived scFv
antibody specific to
Listeria monocytogenes
Xuan-Hung Nguyen
1,2
and
Kim-Anh To
2
1
Toulouse III University, France
2
Hanoi University of Science and Technology, Vietnam
W
e developed a novel simple and cost-effective
immobilization method for bio panning of a phage-
display library. We used Light Expanded Clay Aggregate (LECA)
as biomass support matrix to isolate phage display library-
derived scFv antibody specific to
Listeria monocytogenes
,
a bacterium causing serious illness in human and animal.
Four rounds of positive selection against LECA-immobilized
L. monocytogenes
and an additional subtractive panning
against
L. innocua
were performed. This panning scheme in
combinationwithournovelimmobilizationmethodallowedus
to isolate the phage clones bind to
L. monocytogenes
without
cross-reactivity toward ten other non-
L. monocytogenes
bacteria. One of the selected phage clones was able to
specifically recognize three major pathogenic serotypes
(1/2a, 1/2b and 4b) of
L. monocytogenes
and 11 tested
L.
monocytogenes
strains isolatedfromfoods.ThisscFvantibody
has potential use in development of immunoassay-based
methods for rapid detection of
L. monocytogenes
. In addition,
the LECA-immobilization method described here offers an
efficient, simple and cost-effective bio panning strategy to
isolate specific monoclonal antibodies against any given
species of pathogenic bacteria from phage-display libraries.
e
:
xuan-hung.nguyen@inserm.frMicrobiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-006