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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4
th
International Conference on
Molecular characterization of antibiotic resistant
Escherichia coli
isolates recovered
from food samples and outpatient clinics
Hassan A Hemeg
Taibah University, Kingdom of Saudi Arabia
M
ultidrug-resistant
Escherichia coli
is one of the most
important public health concerns worldwide that can
be transferred through the food of animal origin to human
being causing serious infection. The genetic responsibility of
such resistant genes (Plasmids, integrons and transposons)
can be easily transmitted from the resistant strain to another.
Therefore, the main objectives of the study is the molecular
characterization of the resistant
Escherichia coli
isolates
recovered from food samples and human isolates collected
from outpatient clinics, KSA especially the resistance
strains against aminoglycoside resistance genes which are
responsible for the resistance against gentamicin and the
resistance caused b-lactamases genes. Examination of food
samples revealed 120
Escherichia coli
isolates (22.22%) (30
strains O26: K60,28 strains O128: K67, 20 strains O111: K58,
18 strains O126: K58, 10 strains O55: K59, 9 strains O86: K61
and 5 strains O157: H7). All the strains were highly resistance
to penicillin, amoxicillin-clavulanic and erythromycin with
a percentage of 100%, while the resistance to gentamicin,
ampicillin, oxytetracycline, chloramphenicol, norfloxacin,
trimethoprim, and nalidixic acid were 83%, 75%, 65.3%,
55.8%, 36.5%, 30.7% and 26.9% respectively. On the other
hand, 59.6% of tested strains were sensitive to ciprofloxacin.
Positive amplification of 896 bp fragments specific for aacC2
genes were observed by PCR designated for the detection of
the aminoglycoside resistance genes. Meanwhile, multiplex
PCR designed to detect the ampicillin and amoxicillin-
clavulanic acid resistant
E.coli
isolates revealed positive
amplification of 516 bp fragments specific for BlaTEM gene
with all the resistant strains to ampicillin and amoxicillin
clavulanic acid. Moreover, positive amplification of 392 bp
fragments specific for BlaSHV resistant gene were observed
with (60.52%) of
E.coli
isolate. While all the tested strains
were negative for amplification of BlaOXA_1.
e
:
hasanhemeg@hotmail.comMicrobiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-006