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Journal of Chemical Technology and Applications | Volume 3
MASS SPECTROMETRY,
PROTEOMICS AND POLYMER CHEMISTRY
3
rd
International Conference on
Mass Spectrometry Congress 2019
ANTIMICROBIAL AND DETOXIFYING EFFECTS OF AN EDIBLE BIOPOLYMER POLY
(
γ
-GLUTAMIC ACID)
Bing-Huei Chen
Fu Jen Catholic University, Taiwan
O
wing to an increase in antibiotic-resistant microorganisms and side effects associated with conventional
detoxification chelation therapy, the potential of an edible and biodegradable biopolymer poly(ℽ-glutam-
ic acid) (PGA) was explored as an alternative antimicrobial and detoxifying agent. The PGA-based magnetic
nanoparticles (PGA-MNPs) were synthesized by co-precipitation of ferric chloride and ferrous sulfate using am-
monium hydroxide, followed by without coating (bare MNPs) and 8% coating with sodium salt of PGA (NaPGA)
or 11.8% with calcium salt of γ-PGA (CaPGA). Evaluation of both NaPGA- and CaPGA-MNPs for their antimicro-
bial activity by agar dilution assay showed a lower minimum inhibitory concentration in
Salmonella
Enteritidis
SE 01 than the commercial antibiotics linezolid and cefaclor, but the former was effective against
Escherichia
coli
ATCC 8739 and
Staphylococcus aureus
ATCC 10832, whereas the latter was effective against
Escherichia coli
O157: H7 TWC 01. As a detoxifying agent, PGA showed a rapid adsorption of lead and cadmium at pH 5-8 with
a Langmuir adsorption capacity (LAC) of 98.70 and 31.13 mg/g in deionized water and 147.71 and 23.15 mg/g
in simulated gastrointestinal fluid respectively. Interestingly, the LAC remained unaffected in the presence of
several essential metals such as Cu, Fe, Zn, Mg, Ca and K. Lead and cadmium removal by only PGA also showed
similar results with a LAC of 213.58 and 41.85 mg/g at pH 5.5 respectively. In
in vivo
study, the lead-induced in-
toxication of mice treated with PGA was evaluated by comparing with a standard drug meso-2, 3-dimercapto-
succinic acid. Administration of 200 and 400 mg/kg of PGA reduced the accumulation of lead in liver, heart and
testis with the latter dose being effective in decreasing the lead content in kidney and spleen. Also, the PGA at
both doses reduced TBARs in kidney and brain, elevated δ-aminolevulinic acid dehydrase activity in blood and
decreased the activities of both pyruvic transaminase and lactic dehydrogenase in serum.
J Chem Tech App 2019, Volume 3