6 / 12
Page 48
May 20-21, 2019 | Rome, Italy
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
Journal of Chemical Technology and Applications | Volume 3
MASS SPECTROMETRY,
PROTEOMICS AND POLYMER CHEMISTRY
3
rd
International Conference on
Mass Spectrometry Congress 2019
SYNERGISTIC ERADICATION OF BACTERIAL BIOFILM USING NITRIC OXIDE-LOADED
ANTIMICROBIAL POLYMER
Rashin Namivandi-Zangeneh
University of New South Wales, Australia
T
he formation of bacterial biofilms on the surface of body tissues and medical devices is considered as a crit-
ical healthcare issue causing chronic and recurrent infections. In biofilm mode bacteria cells are enclosed
in a polymeric self-secreted matrix which makes them highly resistant to hostile environmental conditions and
antibiotic treatments. Co-administration of two mechanistically different antimicrobial agents seems to be a
potential approach to overcome challenges in treatment of biofilm-related infections. In this study, Author re-
ports the synthesis of a novel antimicrobial/antbiofilm agent that consists of biocompatible antimicrobial poly-
mer and nitric oxide (NO)-releasing functional groups. The NO-loaded polymer has dual-action capability as it
can release NO which triggers the dispersion of biofilmwhereas the polymer can induce bacteria cell death via
membrane wall disruption. Synergistic antimicrobial activity was observed in biofilm dispersal, planktonic and
biofilm killing activities against Pseudomonas aeruginosa upon the incorporation of (NO)-releasing functional
groups into the structure of the antimicrobial polymer. The NO-loaded polymer results in 80% reduction in bio-
film biomass and kills >99.999% of planktonic and biofilm P. aeruginosa cells within 1hr of treatment at a poly-
mer concentration of 64 µg/ml. To fulfil this synergistic effect, NO donors and antimicrobial polymer should
necessarily exist as a single compound, instead of a cocktail physical mixture of two individual components.
J Chem Tech App 2019, Volume 3