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Journal of Timely Topics in Clinical Immunology | Volume 2
allied
academies
July 26-28, 2018 | Moscow, Russia Immunology
11th Annual Congress on
Tumor liberated protein (TLP) has been previously described
as a TAA (complex) present in the sera from lung cancer
patients with early stage disease.
Since early detection improves overall survival in lung cancer,
identification of screening biomarkers for patients at risk for the
development of this disease represents an important target.
Starting from the peptide epitope RTNKEASI previously isolated
from TLP complexes, we generated a rabbit anti-RTNKEASI
serum. This antiserum detected and immunoprecipitated
a 55kDa protein band in the lysate of the lung cancer cell
line A549. This protein band was identified as aldehyde
dehydrogenase isoform 1A1 through mass spectrometry,
revealing the molecular nature of at least one component of
the previously described TLP complex. Next, we screened a
cohort of 29 lung cancer patients (all histologies), 17 patients
with non-neoplastic lung pathologies and 9 healthy donors for
the presence of serum ALDH1A1 and global serum ALDH by
enzyme-linked immunosorbent assay. This analysis indicated
that the presence of ALDH was highly restricted to patients
with lung cancer. Interestingly, the global ALDH test detected
more lung cancer patients compared to the ALDH1A1-specific
test, suggesting that other ALDH isoforms might add to the
sensitivity of the assay. Our data suggest that ALDH levels may
therefore be evaluated as part of amarker panel for lung cancer
screening.
Finally, the ability of the immune system to recognize a TAA,
enables the development of a vaccine approach for preventive
and therapeutic application and represents a main target of this
field of research.
Speaker Biography
Giulio Tarro graduated from Medicine School, Naples University (1962). Research
Associate, Division of Virology and Cancer Research, Children’s Hospital (1965-1968),
Assistant Professor of Research Pediatrics, College Medicine (1968-1969), Cincinnati
University, Ohio. Oncological Virology Professor, Naples University (1972-1985). Chief
Division Virology (1973-2003), Head Department Diagnostic Laboratories, (2003-
2006). D. Cotugno Hospital for Infectious Diseases, Naples; Emeritus, 2006 -. Since
2007 Chairman Committee of Biotechnologies and Virus Sphere, World Academy
Biomedical Technologies, UNESCO, Adjunct Professor Department Biology, Temple
University, College of Science and Technology, Philadelphia, recipient of the Sbarro
Health Research Organization lifetime achievement award (2010). His researches have
been concerned with the characterization of specific virus-induced tumour antigens,
which were the “finger-prints” left behind in human cancer. Achievements include
patents in field; discovery of Respiratory Syncytial Virus in infant deaths in Naples
and of tumor liberated protein as a tumor associated antigen, 55 kilodalton protein
overexpressed in lung tumors and other epithelial adenocarcinomas.
e:
Giulio Tarro
Foundation de Beaumont Bonelli for Cancer Research, Italy
Tumor liberated protein (TLP) as potential vaccine for Non-Small Cell Lung Cancer
(NSCLC) patients