Previous Page  4 / 15 Next Page
Information
Show Menu
Previous Page 4 / 15 Next Page
Page Background

Page 48

allied

academies

Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4

November 02-03, 2017 | Chicago, USA

Embryology and In vitro Fertilization

World Congress on

Notes:

Generation of cloned adult muscular pigs with myostatin gene mutation by genetic engineering

Xi-Jun Yin

1

, Jin-Dan Kang

1

, Seokjoong Kim

2

, Long Jin

1

, Qing Guo

1

, Xiaoxu Xing

1

, Meifu Xuan

1

, Guanglei Zhang

1

, Qirong Luo

1

, Yong Soo Kim

3

, Chengdu Cui

1

,

Wenxue Li

1

, Zhengyun Cui

1

and

Jin-Soo Kim

4

1

Yanbian University, China

2

ToolGen, Inc., South Korea

3

University of Hawaii, USA

4

Institute for Basic Science, South Korea

B

ecause skeletal muscle is the most economically valuable

tissue in meat-producing animals, enhancing muscle

growth in these species may enhance the efficiency of meat

production. Skeletal muscle mass is negatively regulated

by myostatin (MSTN), and non-functional mutations of the

MSTN gene in various animal species have led to dramatic

hypermuscularity. This study was designed to assess

the characteristics of male MSTN-knockout (KO) pigs. A

transcription activator-like effector nuclease (TALEN) pair

targeting exon 1 of the swine MSTN gene was constructed

and used to transfect porcine fetal fibroblasts (PFFs). We

obtained a cell line that consisting of a 2-bp deletion in one

allele and a 4-bp deletion in the other allele, was used as

a donor to generate cloned pigs via SCNT, and delivered 18

live piglets. They developed and grown normally to sexual

maturity. These MSTN-KO boars grew normally to adulthood

and showed visually-clear hypermuscular characteristics,

increased carcass dressing percentage and loin eye size, and

decreased in backfat thickness. These pigs may show greater

meat production, as well as being used in animal models of

human diseases.

Speaker Biography

Xi-Jun Yin is working as the Director of Jilin Provincial Transgenic Animal and Embryo

Engineering Laboratory at Yanbian University. His research goal is to increase

reproductive efficiency of swine and to expand the genetic potential present in pig

embryos. Recently, his research team successfully produced myostatin gene knockout

double-muscled adult pigs.

e:

yinxj33@msn.com