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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Incidence of embryonic aneuploidy in different age groups of Saudi population undergoing PGS-ICSI
Murid Javed, Othman Abdulrazzak, Fatima Abdelraouf, Tagwa Saad, Sahar Bengawi, Suleiman Najashi
and
Hamad Sufyan
Thuriah Medical Center, Saudi Arabia
Introduction:
Preimplantation Genetic Screening (PGS)
is becoming more frequently employed for detection of
aneuploid embryos to prevent transmission of genetic
defects. A few cells are micro-surgically removed from the
embryo to analyze the DNA, thus selecting embryos with the
highest potential for implantation to optimize a woman’s
chance of healthy live birth and reduce the risk of miscarriage
due to chromosomal aneuploidy. The objective of this study
was to determine incidence of embryonic aneuploidy in
different age groups of Saudi patients undergoing ICSI-PGS
cycles from Jan to Dec 2016 at Thuriah Medical Center,
Riyadh, Saudi Arabia.
Materials & Methods:
Data
from
248
patients
undergoing ICSI-PGS were analyzed. The ovarian
stimulation, oocyte retrieval, ICSI, PGS, culture and transfer
were performed by standard protocols. All sperm samples
were from male partners; 242 fresh ejaculates, 4 frozen-
thawed Micro-TESE and 2 TESA. The embryo biopsies were
performed on day-3 (Fig 1) and analyzed using fluorescence
in-situ hybridization with probes for chromosomes 13, 18,
21, X and Y. The total embryos analyzed were 1055 and
grouped into normal, abnormal, mosaic and undiagnosed.
The patients were grouped into following age categories;
<35, 35-37, 38-40, 41-42 and 43-48 yrs. The statistical
analyses were performed by SPSS.
Results:
The percentages of euploid embryos were; 43,
46, 42, 24 and 28 in <35, 35-37, 38-40, 41-42 and 43-48
yr females, respectively (Table 1). The percentages of
aneuploidy embryos were; 36, 32, 37, 53 and 54 in these
study groups, respectively. The percentages of normal
embryos decreased and percentages of abnormal embryos
increased significantly in females >41 yrs of age. The
percentage of mosaic embryos was significantly higher
in 43-48 yr females. The pregnancy rate was 28, 33, 22, 0
and 25% in <35, 35-37, 38-40, 41-42 and 43-48 yrs age
groups, respectively. In an earlier study of Saudi population
conducted in 2013, the abnormal embryos constituted 36 %
in women with an average age of 34.9 yrs (1). In our study
the % abnormal embryos in < 35 years age group is similar;
however, it increased significantly in women ≥ 41 yrs. Such
data is not available in the previous report.
Conclusion:
The embryonic aneuploidy rate is similar until
age 40, however, it increases significantly in embryos from
41 years or older women.
Speaker Biography
Dr. Javed is Director of ART Laboratories at Thuriah Medical Center, Riyadh, Saudi
Arabia. He has been certified by Canadian Fertility and Andrology Society. He is
member of Practice Committee and Chair of Certification Committee of American
College of Embryology, USA. He is member of many professional societies including
American Society for Reproductive Medicine, Canadian Fertility and Andrology Society
and European society for Human Reproduction and Embryology.His professional
carrier started after obtaining Doctor of Veterinary Medicine Degree in 1981. He
earned MS in Reproductive Physiology in 1984. His initial research experiments were
in Embryo Physiology of research animals. This was the time when this technology
was just beginning in human. In 1986, his curiosity for further knowledge, took
him to Washington State University, USA for PhD in Embryo Physiology. He earned
PhD in 1990. He had the opportunity to earn 2 post doctorate fellowships; first at
Kyoto University, Japan and second at University of Georgia, Athens, USA. At these
institutions, he conducted research on
in vitro
fertilization, embryo culture and
embryo vitrification.
e:
murid.javed@thuriah.com.sa