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June 12-13, 2019 | Edinburgh, Scotland
8
th
European Clinical Microbiology and Immunology Congress
&
3
rd
World congress on Biotechnology
Joint Event
Microbiology: Current Research | Volume: 3 | ISSN: 2591-8036
Preparation of natural plants media for the cultivation of lactic acid bacteria and
pathogens
Duaa S Al-Dulaimy
1
, Basam B Mohammed
2
and
Yusra M B Muhsin
2
1
Cardiff University, UK
2
Mustansiriyah University, Iraq
P
lants play a major role in all the traditional disciplines
of medicine because they are considered as a rich
source of nutrients. The aim of this study is to prepare an
appropriate cultural medium for two groups of bacteria
(lactic acid and pathogens). Three types of plants:
Aloe
vera
, black and green tea were used. For the preparation
of solid media, 100 g/l of each plant were soaked in a
warm water, after that 15 g/l of agar-agar were added
to each of the aqueous extracts. Furthermore, culture
media were prepared by mixing
Aloe vera
with green
tea once and with black tea once again. Genera of lactic
acid bacteria (LAB) used in this research were isolated
from different sources:
Lactobacillus acidophilus
(vagina),
L. casei
(breast milk),
L. paracasei
,
L. plantarum
and
Pediococcus acidilactici
(monkey milk). On the other hands
pathogens were
Serratia
and
Pseudomonas
isolated from
urinary tract infections (UTI) and
Salmonella
isolated
from contaminated food. All test bacteria were cultured
on natural medium and incubated at 37°C for 24 h. Study
results showed that pathogenic bacteria were not able to
grow on black tea medium whereas they could grow on
green tea medium and
Aloe vera
mediumwith few growths
on both. LAB showed obvious growth on two types of
medium green tea and
Aloe vera
, separately. However, this
bacterium could not grow on black tea medium. In terms
of the combined plants media, the medium consisted of
Aloe vera
and black tea did not show any growth for LAB
but there was a growth of all types of pathogens. Both LAB
and pathogens revealed heavy growth on
Aloe vera
plus
green tea medium. In conclusion, green tea and
Aloe vera
is a suitable growth medium for LAB while black tea is not
appropriate for this bacterium. All types of natural media
are suitable for the cultivation of pathogenic bacteria. As a
result of the above findings, it is preferred to use natural
medium instead of chemical medium that have expensive
cost in addition to their negative side effects on human.
Speaker Biography
Duaa S Al-Dulaimy is currently pursuing her PhD in Cardiff University,
UK. She has completed her BSc degree from Department of Biology/
School of Biosciences, Mustansiriyah University, Iraq and her MSc
degree from the same department. She has published several papers
in reputable journals and has been working as a lecturer for more than
ten years teaching the undergraduate students of medical microbiology
and biotechnology. And, she has supervised several undergraduate
students.
e:
Al-Dulaimyds@cardiff.ac.ukDuaa S Al-Dulaimy et al., Microbiol Curr Res, Volume 3
ISSN: 2591-8036