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Journal of Medical Oncology and Therapeutics | Volume: 3
July 23-25, 2018 | Moscow, Russia
12
th
World Cancer Congress
Features of HPV infection in squamous cell cervical carcinomas of Immunocompromised patients
Elizaveta Starodubova
3
, Runov A L
1
, Savostjanov T F
2
, Kurchakova E V
1
, Isaguliants M G
3
, Albegova L E
4
, Demenkova M A
4
,
and
Vonsky M S
1
1
Institute of Cytology of Russian Academy of Science, Russia
2
City Clinical Oncological Dispensary, Russia
3
N. F. Gamaleya Federal Research Center, Russia
4
City Tuberculosis Hospital, Russia
P
ersistence of high carcinogenic risk (HR) HPVs in
immunocompetent patients is limited by effective anti-
viral immune response, while immune suppression leads to
persistence of HR-HPV types, eventual transformation, and
development of squamous cell cervical carcinomas (SCCC) with
alterations inexpressionof check-point inhibitor genes including
PD-1
and
PD-L1
. We analyzed distribution of HR-HPV types, and
PD-1
and
PD-L1
gene expression in SCCC tissues of patients
immunocompromised by HIV-1, mycobacterial/TB infections,
and HIV/TB-coinfection. Study included 10 HIV(-)TB(-), 8 HIV(-)
TB(+), 9 HIV(+)TB(-) and 13 HIV(+)TB(+) patho-morphologically
verified SCCC cases. Total DNA and RNA was extracted from
FFPE samples using All Prep DNA/RNA FFPE kit (Quiagen).
Presence of 12 HR-HPV types was determined by quadruplex
real-time PCR (AmpliSense HPV HOR genotype FL).
PD1
and
PD-L1
expression was assessed by real-time PCR after reverse
transcription. HPV16, 18 and 33 were found in all; HPV39 in
HIV(+)TB(-); HPV31, 35, 39, 52 inHIV(-)TB(+); andHPV33, 35, 39,
45, 52, 56, 59 in tumors of HIV(+)TB(+)-patients. HIV(+) samples
were characterized by relatively decreased expression of
PD-1
(76%) and increased expression of
PD-L1
(151%). Expression of
PD-L1
tended to correlate with HPV18 load (R=0,69; p=0.08). In
conclusion, immunosuppression due to HIV-infection results in
increased abundance in SCCC tissues of HR-HPV of types other
than 16, 18 and 33, and immunosuppression due to HIV/TB-
coinfection, to additional increase in the number of circulating
HR-HPV types. HR-HPVsmodulate the level of PD-L1 expression.
Significance of PD-1/PD-L1 findings would be verified after
complementation of the cohort with additional samples.
Speaker Biography
Elizaveta Starodubova has completed her PhD in the Engelhardt Institute of Molecular
Biology, Russian Academy of Sciences, and continued her postdoctoral studies there
and at the Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska
Institutet, Stockholm. She performs her studies in the field of antigen processing and
design of prototype DNA-vaccines against viral infections and cancer.
e:
estarodubova@gmail.com