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November 20-21, 2019 | Berlin, Germany
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
ANALYTICAL CHEMISTRY AND
CHROMATOGRAPHY METHODS
2nd International Conference on
Analytical Chemistry 2019
ANTI-CANCER ACTIVITY AND IMMUNOADJUVANT PROPERTIES OF EDIBLE BIRD’S
NEST EXTRACTS ON HUMAN BREAST CANCER CELL LINE
Lee Ting Hun, MYoshiny and S Maruthai
Universiti Teknologi Malaysia, Malaysia
Edible Bird’s Nest (EBN) is well regarded as an immune enhancing nutraceutical food especially among the
Chinese. Current cancer therapies bring adverse side effects, thus identification of a safe adjuvant medi-
cine like EBN can provide a quality life for patients. The aim of this study is to evaluate the anti-cancer activi-
ty and immunoadjuvant properties of EBN extract on human breast cancer cell line (MCF-7). Primarily, three
types of EBN extracts coded as HMG, EHMG and pHMG were prepared using the water extraction method.
These EBN extracts were then tested on their cytotoxicity level against MCF-7 and human immune cells (CD8+
and CD14+). Production of the key pro-apoptotic and anti-apoptotic molecules released in MCF-7, CD8+ and
CD14+ cells before and after EBN treatment were measured through mRNA expression level, ELISA and Multi-
plex assay. Among the three EBN extracts, HMG showed the highest cytotoxic effect towards MCF-7 cells with
IC50 of 15µg/mL. However, HMG showed no harm towards CD8+ and CD14+ cells with cell viability of more
than 90%. qRT-PCR results for activated CD8+ and CD14+ cells showed increased of pro-apoptotic gene ex-
pression after treated with HMG in co-culture. At the same time, supplementation of HMG increased the apop-
tosis through down regulation of anti-apoptotic genes and the up-regulation of pro-apoptotic genes in MCF-7
cells. Enhancement of pro-apoptotic and down regulation of anti-apoptotic soluble factors by non-activated
and activated CD8+ and CD14+ cells in single and co-culture after treated with HMG also showed in ELISA and
multiplex assay. In conclusion, the present study showed that HMG extract is a potential anti-cancer agent and
causes no harm to human immune cells. qRT-PCR, ELISA and multiplex tests also verified that HMG acts as an
immunoadjuvant by enhancing pro-apoptotic function in the human immune cells.
Lee Ting Hun et al., J Chem Tech App 2019, Volume 3
Lee Ting Hun is a senior lecturer and the Head of Edible Bird’s Nest Unit in School of Chemical Engineering, Universiti Teknologi
Malaysia. He is the pioneer in carrying out bird’s nest and swiftlet ranching research in Malaysia. He has led 20 over numerous gov-
ernment and private sector projects amounting to more than RM2 million. He has also published 30 over-indexed journals papers,
conferences and seminars papers, book and book chapter. For decades, he has invented numerous processes and innovate some
noble products related to bird’s nest, nutraceutical and cosmeceutical. His contribution to the industry has won him the Malaysian
Scientist Award and the Geneva Invention and Innovation Award. He has also co-authored the Malaysian SIRIM standards for Edible
Bird’s Nest and Swiftlet Ranching Standards. Recently, he has also been appointed as Malaysia Bird’s Nest Expert.
BIOGRAPHY
Journal of Chemical Technology and Applications | Volume 3