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April 15-16, 2019 | Milan, Italy
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Ortho Congress 2019
Journal of Orthopedic Surgery and Rehabilitation | ISSN: 2630-4473 | Volume 3
ORTHOPEDICS, RHEUMATOLOGY
AND OSTEOPOROSIS
2
nd
Annual Conference on
TREATMENT OF STABLE VITILIGO USING BOTH CULTURED AND NON-CULTURED
AUTOLOGOUS MELANOCYTES FROM HAIR FOLLICLE ORS CELL SUSPENSION
Shereen Mahmoud Kamel Shawky
1, 2
1
Cairo University, Egypt
2
Al Kasr Al Ainy hospitals, Egypt
Background:
Treatment of stable vitiligo is mainly surgical. A plethora of methods are being studied and mod-
ified, however none of them guarantee 100% cure up till now. Arguments existed about the safety, efcacy and
convenience of cultured melanocytes as compared to non-cultured technique. In 2012, Savkovic and Co-work-
ers developed an improved culture method amplifying human melanocytes from the hair follicles; however,
they did not apply this method clinically for treating vitiligo patients.
Aim:
To assess the efcacy and safety of a modified autologous cultured hair follicle outer root sheath cell
suspension transplantation in the treatment of stable vitiligo lesions, and to compare it with the results of
transplantation of autologous non-cultured hair follicle cell suspension within the same patient.
Patients & Methods:
Hair samples were epilated from 24 patients with stable vitiligo (each sample 60-80 an-
agen hairs), hair follicle ORS was separated by two methods, cultured [we modified the culture method of
Savkovic et al. (2012)] and non-cultured methods. Melanocytes characteristics in both methods were deter-
mined via measuring cellular melanin content by ELISA and the fold change in pre-melanosome (Pmel-17)
gene expression. Patients’ response was evaluated clinically for up to one year following treatment.
Results:
Out of 24 patients, 19 patients completed the study, with a total of 40 lesions treated, 21 with cultured
and 19 with non-cultured melanocyte cell suspension and continued the follow-up period. Eight (20%) lesions
showed excellent response (2 (25%) of them with non- cultured and 6 (75%) with cultured), 10 (25%) good (3
(30%)of them with non- cultured & 7 (70%) with cultured), 7 (17.5%) fair (3 (43%) of them with non- cultured
and 4 (57%) with cultured), and 15(37.5%) showed poor response (11(73%) of them with non- cultured and 4
(27%) with cultured). Melanin content and melanocyte viability were more with the modified cultured tech-
nique.
Conclusion:
Our provided modified autologous hair follicle ORS melanocytes cultured cell suspension trans-
plantation method is a promising option for treating stable vitiligo lesions, however it is more expensive and
time consuming than the non-cultured method. A plethora of interventions had been introduced for treating
stable vitiligo lesions, however none could guarantee 100% cure. Arguments existed about the safety, efca-
cy and convenience of cultured melanocytes as compared to non-cultured technique. In 2012, Savkovic and
Co-workers developed an improved culture method amplifying human melanocytes from the hair follicles;
however, they did not apply this method clinically for treating vitiligo patients. We assessed the efcacy and
safety of modified autologous cultured hair follicle outer root sheath cell suspension transplantation in the
treatment of stable vitiligo lesions, in comparison to autologous non-cultured hair follicle cell suspension
transplantation within the same patient. The modified cultured method seems to be promising although more
expensive and more time consuming than the non-cultured technique.
Shereen Mahmoud Kamel Shawky, J ortho Rehab Surg. 2019, Volume 3 | DOI: 10.4066/2630-4473-C1-002