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Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Notes:
Page 42
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
MOLECULAR DIAGNOSIS FOR THE RAPID
DETECTION OF BOVINE TUBERCULOSIS IN
THE STATE OF KUWAIT
Al Mouqatea S A
Kuwait Institute for Scientific Research, Kuwait
Mycobacterium bovis is the causative agent of bovine
tuberculosis (bTB), a zoonotic disease with an
overall negative impact on the livestock industry. TB has
been reported in Kuwait. Because of the adverse social and
economic impact that the disease imposes on livestock and
the people of Kuwait, development of surveillance, diagnostic,
and control programs are needed to detect new cases and
eradicate the disease. Hundred and four dairy cattle tested
using the universally accepted comparative intradermal
tuberculin test (CITT) was the primary test used during the
survey work for assessing the prevalence of bTB in Kuwait’s
dairy herds. Rapid and highly sensitive molecular diagnostic
tools, such as DNA Extraction from blood real-time PCR
(polymerase chain reaction) and ELISA (enzyme-linked
immunosorbent assay), have been evaluated and compared
with traditional, delayed hypersensitivity- and slaughterhouse
inspection-based diagnostic schemes. The total number of
cases detected between 2012 and 2015 in 10 cattle farms in
the state of Kuwait was 104 positive TB cases, which had a
mean prevalence of 2.1% per farm. Highest numbers of
cases were detected in February 2015, with no seasonal
patterns inferred. Spearman correlation coefficients and their
corresponding p-values between disease status and both farm
size (p=0.43, p-value=0.032) and agricultural area (p=0.49,
p-value=0.015) were significant at the 95% confidence
level. The overall hierarchal mixed-effect logistic regression
analysis was significant (p-value=0.0413). As expected, our
results suggested that the prevalence of TB detected cases
didn’t follow any seasonal patterns, first because, TB is a
chronic disease and seasonality can’t be quantified within
4 years of surveillance efforts. Second, case detection was
highly dependent on the intensification of sample collection
at a given season, in which the number of collected samples
was substantially high in winter and low in summer seasons.
Microbiology: Current Research 2017