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allied
academies
J u n e 2 8 - 2 9 , 2 0 1 8 | A m s t e r d a m , N e t h e r l a n d s
Joint Event on
OBESITY AND WEIGHT MANAGEMENT
VACCINES AND IMMUNOLOGY
&
International Conference on
International Conference on
Asian Journal of Biomedical and Pharmaceutical Sciences
|
Volume 8
ISSN:
2249-622X
ASSOCIATION OF CARRIERS OF
LEPR Q223R
WITH LEVEL
OF THYROID HORMONES IN FEMALE ADOLESCENTS WITH
OVERWEIGHT AND OBESITY
Tatyana Bairova, Ievleva K, Sheneman E
Hramova E, Rychkova L
and
Kolesnikova L
Scientific Center for Family Health Problems and Human Reproduction, RF
Introduction: Hypothalamus is the center of regulation of homeostasis, including energy homeostasis. Data on the relationship of
obesity, hyperleptinemia and thyroid dysfunction are discrete, especially in conditions of an ontogenetically determined change in
the hypothalamus sensitivity threshold. Previous studies indicate the relationship between hyperleptinemia and polymorphism of
the leptin receptor gene (LEPR) in obese patients.
Aim: To determine association of
LEPR Q223R
with level of thyroid hormones in female adolescents with overweight and obesity.
Materials &Methods: A total of 114 Caucasian female adolescents was observed (average age 15.80±1.08 years): the main group
included 68 girls with overweight and obesity (SDS BMI 2.81±0.64) and the control group included 59 girls (SDS BMI 0.34±0.59).
Serum thyroid-stimulating hormone (TSH) and free thyroxine (free T4) levels were measured using commercially available
enzyme-linked immune absorbent assay (DBC, Canada) with absorbance microplate reader ELx808 (Biotek, USA). Genomic DNA
was extracted from whole blood by commercial kits. Genotyping
LEPR Q223R
was performed using polymerase chain reaction
with electrophoresis detection.
Results: TSH level was significantly higher in main group (2.085±1.201 mcED/ml) than in control group (2.055±0.766 mcED/ml)
(p=0.0349). T4 free level was 12.955±2,495 pM/ml in control group and 13.113±2.980 in main group (p=0.8672). In control group,
TSH and free T4 levels in carriers of different genotypes of
LEPR Q223R
were: 3.089±4.110 mcED/ml and 11.883±3.000 pM/ml in
RR-carriers and 1.250±0.474 mcED/ml 14.267±1.556 in QQ-carriers, respectively (p=0.0143; p=0.0150). There is no association
of R-allele carriers with TSH and T4 free levels in adolescents with overweight and obesity.
Conclusion: Increase of TSH level and normal T4 free level were shown in adolescents with overweight and obesity. This indicates
subclinical hypothyroidism. Carrier of the risk R-allele associated with increase of the TSH level in girls with normal weight, but
not in girls with overweight and obesity.
asiy91@mail.ruAsian J Biomed Pharmaceut Sci 2018, Volume 8 | DOI: 10.4066/2249-622X-C1-003