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J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y
Cardiology Congress 2018 & Microbe Infection 2018
Note:
Page 27
allied
academies
2
nd
World Congress on
CARDIOLOGY
MICROBIOLOGY AND MICROBIAL INFECTION
&
39
th
Annual Congress on
Joint Event on
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
Ebtesam Al-Ali, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C1-001
DETECTION OF TYLCV ON CUCUMBER
CROPS IN KUWAITI FARM
H
igh scores of vegetable crop losses were recorded in Kuwait agricultural
farms, viral diseases were the main causal agent of these economic losses
in many crops, mainly in tomato and recently recorded in cucumber. Tomato
yellow leaf curl virus (TYLCV) was reported as a major pest of tomato and
cucumber but it was not characterized at the molecular level. The whitefly
was the main transmitter of TYLCV. Common symptoms on cucumber plants
infected with TYLCV were: Leaf and fruit deformation, mosaicing, yellowing,
upward leaf cupping, and stunting. Two hundred samples of cucumber leaves
were collected, and the symptoms resulting from viral diseases were recorded
and documented. DNA was extracted from 300 infected cucumber leaf
samples, and PCR detectionwas performed on 150 samples using two different
primer pairs (TY1 and TY2 and TYC1R and TYC1F). PCR tests revealed that 80
samples out of 150 tested samples were positive. Best results were performed
by TY1 and TY2 primer pair. Positive samples were stored for further analysis.
Biography
Ebtesam Al-Ali has obtained her BSC in 1993, from
Kuwait University and worked for Kuwait Univer-
sity as Research Assistant, then joined KISR and
led eight projects. She has published more than 30
papers in reputed journals and international con-
ferences. Her field of experiences is in plant virus
detection, primer design, cloning and sequencing,
ELISA, DNA extraction, PCR amplification, RCA
rolling circle amplification, TYLCV detection on
tomatoes, also trained twice in the University of
Wisconsin Madison under the supervision of Prof.
Amy Charkowski as well as University of Washing-
ton state under supervision of Prof. Hanu Pappu.
ebtisam_alali@hotmail.comEbtesam Al-Ali
Environmental and Life Sciences
Research Centre, Kuwait