Aim: To evaluate the effect of Schisandra chinensis seed extract (used in Chinese medicine) on cisplatininduced cytotoxicity in osteosarcoma cells.
Methods: MTT and caspase assays were used to determine the apoptotic potential of cisplatin and Schisandra chinensis seed extract on Human Osteosarcoma Cells (HOS). The effect of exposure to cisplatin and Schisandra chinensis extract on the expression of caspase 3 was evaluated. The sensitivity of osteosarcoma cells to cisplatin and the expression of phosphor glycoprotein and cisplatin separately and in combination with Schisandra chinensis extract were assessed.
Results: The results of the study indicate cisplatin and seed extract of Schisandra chinensis induced apoptosis in human osteosarcoma cells. The combination of cisplatin and seed extract produced a significant increase in the apoptosis level in osteosarcoma cells. Caspase activation increased significantly on treatment with cisplatin and of Schisandra chinensis seed extract. The seed extract increased the cisplatin sensitivity as well as the expression of phosphoglycoprotein.
Conclusion: Schisandra chinensis seed extract ameliorates cisplatin-induced cytotoxicity through apoptosis and increases sensitivity by increasing the expression of p-glycoprotein.