Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 12

The effect of wulongdan on neuroinflammation factors and the expression of P2X7 receptor in the SAH rats

Objective: To investigate the effect of Wulongdan on the expression of P2X7 receptor (P2X7R) in the cerebral cortex and on neuroinflammation after Subarachnoid Hemorrhage (SAH) in rats.

Methods: A reliable SAH model was established by double injections of blood into cisterna magna in rats. Fifty-four adult Sprague-Dawley rats weighing 220 plus or minus 20 g were divided into three groups by randomized sorting method: Sham, SAH and Wulongdan (n=18 each). The levels of Tumor Necrosis Factor alpha (TNF-α) and Interleukin-1β (IL-1β) were measured by ELISA analysis. The expression of P2X7R in the cerebral cortex was tested by immunohistochemical and Western blot methods.

Results: Compared with those in the Sham group, the levels of TNF-α and IL-1β in the cerebral cortex were significantly up-regulated at each time point after SAH (P<0.05), with the peak occurring at 24 h. Compared with SAH group, the expressions of TNF-α, IL-1β and P2X7R were down-regulated in Wulongdan-treatment group (P<0.05).

Conclusion: Wulongdan could attenuate neuroinflammation in the cerebral cortex after SAH in rats, which may be associated with the down-regulation of P2X7R protein.

Author(s): Chuanwu Zhu, Fang Yang, Rongyan Jiang

Abstract Full Text PDF