Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 16

Role of Wnt/β-catenin signaling pathway in migration of asthmatic rat airway smooth muscle cells

Objective: To investigate the role of Wnt/β-catenin signaling pathway on migration ability in Asthmatic Rat Airway Smooth Muscle Cells (ASMCs).

Methods: The asthmatic rat airway smooth muscle cells were cultured in vitro. The expression of Wnt/β- catenin in ASMCs was detected by RT-PCR and Western blot. Migration ability of ASMCs was detected by wound healing assay and Transwell migration assay.

Results: Compared with normal rats, the expression level of Wnt/β-catenin in chronic asthmatic rats ASMCs was obvious higher. Knockdown of Wnt/β-catenin signaling pathway by specific siRNA was able to decreases the migration ability of ASMCs.

Conclusion: The migration ability of ASMCs of chronic asthma rats’ increases, and Wnt/β-catenin signaling pathway may participants in this event.

Author(s): Xiaomei Fang, Jianjiang Zhao, Lina Shen, Miaofen Zheng, Hong Zhou, Guofang Tao, Zheng Wu, Yuhua Ge

Abstract Full Text PDF