Biomedical Research

- Biomedical Research (2015) Volume 26, Issue 1

Role of Chloride Ion Channels in Human Lens Epithelial Cell Proliferation, Adhesion, and Migration.

The aim of this study was to investigate the proliferation, adhesion, and migration function of chloride ion channels in HLEC B-3, a human lens epithelial cell line, and provide experimental basis for studying the mechanism of human after-cataract as well as the effective methods for controlling and treating it. MTT and Scratch assay methods were applied to detect the proliferation, adhesion, and migration of HLEC B-3 after applying 5, 10, 20, 50, 100, and 200 μmol/L of chloride ion channel blockers (NPPB). In the cell proliferation experiment, no apparent difference was found in the first 12 and 24 h after treatment with 10, 20, 50, 100 and 200 μmol/L NPPB or 48 h after treatment with 20, 50, 100 and 200 μmol/L NPPB. The cell proliferations were obviously inhibited compared with the control group(without NPPB treatment), and the difference was significant (P < 0.05 or P < 0.01). Cell adhesion experimental results showed that when the cells were treated with 10, 20, 50, 100, and 200 μmol/L NPPB for 12 or 24 h, cell adhesions were obviously inhibited. The difference was very significant (P < 0.05 or P < 0.01) compared with the control group. The activity of chloride ion channels can inhibit the proliferation, adhesion, and migration of LECs; therefore, inhibiting its activity can control the occurrence of human after-cataracts.

Author(s): Gui-Rong Li, Qiong-Shu Li, Xin-Yue Yang, Miao-Miao Liu, Yue Cheng, Rui Fei, Ya-Ping Li

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