In this study sputum culture positive cases for tuberculosis in automated TB culture system were studied for patients with occupation related to silica and for patients with occupation not related to silica and their important related findings were categorized and analysed. Similarly control groups were also studied who were TB culture negative in automated TB culture system. Tuberculosis was found common in the age group of 21-30 years, but in patients with silica related occupation, it also occurred at a higher age group and in silica group there is a male preponderance. Among associated microorganisms Candida spp. were found more in silica group than in non-silica group. The growth pattern of Mycobacteria spp. Indicated a definite role of silicon on multiplication and growth of Mycobacterium spp.