Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +44-7360-538437

Research Article - Biomedical Research (2019) Volume 30, Issue 6

Paclitaxel induces apoptosis in osteosarcoma via promoting the production of reactive oxygen species and inhibiting the expression of cyclooxygenase-2

Objective: The present study was designed to investigate the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Study design: Cell viability was detected in human osteosarcoma cell line HOS-732 treated with paclitaxel at different concentrations. The apoptosis and reactive oxygen species (ROS) production rates were determined by flow cytometry. COX-2 expression at mRNA and protein levels in osteosarcoma tissues were measured by quantitative real time polymerase chain reaction (RT-PCR) and Western blotting. Results: Paclitaxel can obviously inhibit the proliferation of HOS-732 cells in a dose-dependent manner. The production of ROS in HOS-732 cells was remarkably increased with the increasing concentration of paclitaxel. Moreover, paclitaxel can significantly inhibit the expression of COX-2 at mRNA and protein levels in rat osteosarcoma tissue. Furthermore, the inhibitory effects of paclitaxel on COX-2 expression at mRNA and protein levels were dose-related. Conclusion: Paclitaxel treatment can induce the apoptosis and increase the production of ROS in HOS-732 cells. Moreover, paclitaxel can decrease the expression of COX-2 in a dose-dependent manner. These findings suggested that paclitaxel-induced apoptosis in osteosarcoma could be ascribed to the increased production of ROS and the reduction of COX-2 expression.

Author(s): Ming Li, Liming Yin, Lili Wu, Yunsen Zhu, Xi Wang

Abstract PDF

Get the App