Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +44-7360-538437

Research Article - Biomedical Research (2017) Volume 28, Issue 11

Molecular mechanism of microRNA-93 in suppressing the cell proliferation of cervical cancer by adjusting the EGFR/AKT signal pathway

Purpose: This study aims to discuss and explore the influences of microRNA-93 on the cell proliferation of cervical cancer and its regulation mechanism.

Methods: Constructed microRNA-93 expression vectors were transfected into the HeLa cell line through lipofection, after which the expression of microRNA-93 in HeLa cells was detected through qRT-PCR. In addition, the influences of microRNA-93 on HeLa cell proliferation were detected through CCK-8. Western blot was used to analyse changes of the EGFR/AKT signal pathway in Hela cells after the overexpression of microRNA-93.

Results: Compared with the control group, microRNA-93 was effectively heterologously expressed in HeLa cells. The CCK-8 results demonstrated that heterologous overexpression of microRNA-93 obviously suppressed Hela cell proliferation. Western blot showed that heterologous overexpression of microRNA-03 inhibited the expression of the EGFR/AKT signal pathway.

Conclusions: The heterologous overexpression of microRNA-03 may inhibit the proliferation of cervical cancer cells. Hence, microRNA-93 can regulate the proliferation of cervical cancer cells by suppressing the EGFR/AKT signal pathway.

Author(s): Xue Dong, Yan Wang

Abstract Full Text PDF

Get the App