Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 11

Effects of 1, 25-dihydroxyvitamin D3 on expression of TGF-β1, CD68 and MCP-1 in type 2 diabetic nephropathy rat

Objective: This study aims to investigate the role and mechanism of 1, 25-dihydroxyvitamin D3 in type 2 diabetic nephropathy rats.

Methods: There were 30 male Sprague-Dawley (SD) rats that were randomly divided into 3 groups: the normal control group (NC group, n=10), the diabetic nephropathy model group (T2DN model group, n=10), and the diabetic nephropathy group treated with 1, 25-dihydroxyvitamin D3 (vD3-T2DN group, n=10). After 15 weeks, the changes of renal tissues morphology, renal function, and 24 h urinary protein quantification were measured. The expressions of Transforming Growth Factor beta 1 (TGF-β1), CD68 and Monocyte Chemoattractant Protein-1 (MCP-1) in renal cortex were also detected by Immunohistochemistry (IHC).

Results: The weight of rats were significantly decreased in T2DN model group and vD3-T2DN group than in NC group (P<0.05) at the end of 15th week, while the blood glucose, 24 h urinary protein and triglyceride were significantly increased (P<0.01). The expression of TGF-β1, CD68 and MCP-1 in T2DN model group and vD3-T2DN group were significantly higher than in NC group (P<0.01). Compared with NC group, the triglyceride (P<0.01) and serum creatinine (P<0.05) were significantly higher in T2DN model group. In vD3-T2DN group, the expression of TGF-β1, CD68 and MCP-1, the content of 24 h urinary protein (P<0.01), and triglyceride (P<0.05) were significantly than in T2DN model group.

Conclusion: Through repressing the expression of TGF-β1, CD68 and MCP-1, 1, 25-dihydroxyvitamin D3 can inhibit invasion of macrophages to protect kidney of T2DN rats.

Author(s): Xiaoyun Zeng, Fapeng Li, Li Quan, Hua Yao, Jun Zhu

Abstract Full Text PDF