The current investigation deals with the potential anti-mycobacterial worth of andrographolide against Mycobacterium microti, commonly known as vole tuberculosis, employing different techniques viz. zone of inhibition, disc diffusion and turbidometric method. The bioactive compound, andrographolide, was isolated from the leaves of Andrographis paniculata. The MIC of andrographolide was found to be vacillating between 100 and 11.11μg/ml against the test organism. The results suggested andrographolide compound to be a potent antibiotic against M. microti and its inhibitory effect is on par with standard drugs like isoniazid and fluoroquinolones.